報告題目:Quantitative modeling of microRNA-mediated regulation on competing endogenous RNAs
報告人: 汪小我,清華大學自動化系、清華信息科學與技術國家實驗室副教授
主持人:蔡軍 研究員
時 間:2015年4月13日(星期一)下午2:00-3:00
地點: 基因組所 5層會議室
報告人簡介:汪小我,分別于2003年和2008年在清華大學自動化系獲得工學學士學位和工學博士學位,現任清華大學自動化系、清華信息科學與技術國家實驗室副教授。主要研究方向為microRNA調控、高通量測序數據分析方法、細胞衰老機制的系統生物學研究等。曾獲全國優秀博士論文獎,并獲得教育部新世紀優秀人才支持計劃和國家自然科學基金優秀青年基金支持。
報告內容簡介:Competing endogenous RNAs (ceRNAs) cross-regulate each other at post-transcriptional level by titrating shared microRNAs (miRNAs). We established a minimum ceRNA model to quantitatively analyze the behavior of the ceRNA regulation and implemented multi-fluorescent synthetic gene circuits in cultured human cells to validate our predictions. Our results suggested that the ceRNA effect is affected by the abundance of miRNA and ceRNAs, the number and affinity of binding site, and the mRNA degradation pathway determined by the degree of miRNA-mRNA complementarity. Furthermore, we found that a non-reciprocal competing effect between partial and perfect complementary targets is mainly due to different miRNA loss rate in these two types of repressions, which sheds light on utilizing such a competing model for rational design of effective siRNA.
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