報告題目：Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity

　　報告人：Xingqi Chen 瑞典卡羅林斯卡醫學院助理教授

　　主持人：李明錕 研究員

　　時間：2018年11月8日（星期四）上午10：00-11：30

　　地點：中國科學院北京基因組所一樓會議室

　　摘要：

　　Here we introduce Protein-indexed Assay of Transposase Accessible Chromatin with sequencing (Pi-ATAC) that combines single-cell chromatin and proteomic profiling. In conjunction with DNA transposition, the levels of multiple cell surface or intracellular protein epitopes are recorded by index flow cytometry and positions in arrayed microwells, and then subject to molecular barcoding for subsequent pooled analysis. Pi-ATAC simultaneously identifies the epigenomic and proteomic heterogeneity in individual cells. Pi-ATAC reveals a casual link between transcription factor abundance and DNA motif access, and deconvolute cell types and states in the tumor microenvironment in vivo. We identify a dominant role for hypoxia, marked by HIF1aprotein, in the tumor microvenvironment for shaping the regulome in a subset of epithelial tumor cells.